How to use the report?

Summary

Unique Genes: 656
Unique Isoforms: 3925

Gene classification

Isoform Classification

Splice Junction Classification

Gene Characterization

Number of Isoforms per Gene

Distribution of Isoforms per Gene

Kown vs Novel Genes

Transcript Length

Classification

Normalized Classification

All Transcripts

By Structural Category

Mono- vs Multi-Exons

Exon Structure

Quantification

Gene Expression, Annotated vs Novel

Structural Isoform Characterization

Isoform Distribution

Across Structural Categories

Across FSM

Across ISM

Across NNC

Across NIC

Across Genic Genomic

Across Antisense

Across Fusion

Across Intergenic

Across Genic Intron

Transcript Lengths

By Structural Classification

By Subcategory

Exon Counts by Structural Classification

By Structural Classification

By Subcategory

Supported Transcript Models

Structural Classification

Structural Subcategories FSM and ISM

Structural Subcategories NIC and NNC

Matched Reference Transcripts

Length Distribution

Exon Count Distribution

Transcript Expression

By Structural Category

By Subcategory FSM/ISM

By Subcategory NIC/NNC

By Subcategory Other

Splice Junction Characterization

Distribution

Distribution of Splice Junctions by Structural Classification

Distribution of Transcripts by Splice Junctions

RT−Switching

RT−Switching All Junctions

Unique Junctions RT−switching

Comparison With Annotated TSS and TTS

Distance to Annotated Polyadenylation Site

For FSM

For ISM

Distance to Annotated Transcription Start Site

For FSM

For ISM

Comparison With Annotated TSS and TTS by Subcategories

Distance to Annotated Polyadenylation Site

Alternative 3’End for FSM

Alternative 3’5’End for FSM

Alternative 5’End for FSM

Reference Match for FSM

3’ Fragment for ISM

Internal Fragment for ISM

5’ Fragment for ISM

Intron Retention for ISM

Distance to Annotated Transcription Start Site

Alternative 3’End for FSM

Alternative 3’5’End for FSM

Alternative 5’End for FSM

Reference Match for FSM

3’ Fragment for ISM

Internal Fragment for ISM

5’ Fragment for ISM

Intron Retention for ISM

PolyA Distance Analysis

PolyA Distance Analysis by categories

PolyA Distance Analysis by categories (table)

PolyA Distance Analysis by subcategories

PolyA Distance Analysis by subcategories (table)

Multi-exonic FSM

All FSM

Subcat: Alternative 3’ End

Subcat: Alternative 3’5’ End

Subcat: Alternative 5’ End

Subcat: Reference Match

Multi-exonic ISM

All ISM

Subcat: 3’ Fragment

Subcat: Internal Fragment

Subcat: 5’ Fragment

Subcat: Intron Retention

Multi-exonic NIC

All NIC

Subcat: Combination of Annotated Junctions

Subcat: Combination of Annotated Splice Sites

Subcat: Intron Retention

Multi-exonic NNC

All NNC

Subcat: Combination of Annotated Junctions

Subcat: Combination of Annotated Splice Sites

Subcat: Intron Retention

Subcat: Mono-Exon by Intron Retention

Subcat: At Least One Annotated Donor/Acceptor

CAGE Detected (table)

Redundancy Analysis

Only FSM

Only FSM

FSM with a polyA motif found

Only ISM

Only ISM

ISM with a polyA motif found

FSM and ISM

FSM and ISM

FSM and ISM with a polyA motif found

Intra-Priming Quality Check

By Structural Category

Mono- vs Multi-Exon

Coding vs Non-Coding

Features of Bad Quality

Quality Control Attributes Across Structural Categories

Quality Control Attributes Across Structural Categories

RT-switching

Non-Canonical Junctions

Splice Junctions Without Short Read Coverage

Nonsense-Mediated Decay by Structural Category

Features of Good Quality

TSS and TTS Support Percentages

Annotation support

CAGE Support

PolyA support

SJ Support Percentages

Isoforms all canonical

SJ with coverage

Attributes

Good Quality Control Attributes

ratio TSS